A simple and rapid method for intra- and interspecific transformation of Bacillus subtilis on solid media by DNA in protoplast lysates |
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| Authors: | Akamatsu T Taguchi H |
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| Institution: | Department of Applied Microbial Technology, Sojo University, Kumamoto, Japan. akamatsu@bio.sojo-u.ac.jp |
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| Abstract: | A simple method for intra- and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3 x 10(3) transformants per microg DNA, 10-20 times higher than that for conventional transformation on solid media. The method can be applicable to transformation in microtiter plates. |
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