| Abstract: | A-decomposing enzyme preparation (α-N-acetylgalactosaminidase) from hog liver, liberates from the lipopolysaccharide of Salmonella riogrande, which contains O antigen 40, 15.5% of its original amount of N-acetylgalactosamine, destroying its ability of inhibiting the agglutination of human A red ceils by anti-S. riogrande rabbit serum. From the structures of blood group A-active oligosaccharides, obtained from the lipopolysaccharide by mild acid-hydrolysis, it was assumed that the antigenic determinant of A specificity might have the following structure, which indicated the presence of N-acetylgalactosaminyl residue as the non-reducing end; α-N-acetylgalactosaminyl-(1→3)-mannosyl-glucosyl-(1→3)-N-acetylgalactosaminyl-glucose. |
