The relative merits of direct morphometry of reconstructions of whole cells,and statistical morphometry by stereology of random sections of cells |
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Authors: | Donald F Parsons Michael Marko Ardean Leith |
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Institution: | 1. Wadsworth Center for Laboratories and Research, New York State Department of Health, Empire State Plaza, PO Box 509, 12201-0509, Albany, NY
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Abstract: | Stereology, or the derivation of quantitative, three-dimensional (3-D) data about cells by statistical analysis of the structures
of random sections, is widely used in cytology and pathology. However, there are situations where this approach is inadequate,
and only an analysis of a homogeneous population of whole cells will give the required results. This involved 3-D reconstruction
from physical or optical sections, or tomography or photogrammetry of whole-cell mounts. Use of stereo views of individual
sections or projections adds considerably to the information available for both contouring and reconstruction. Recent image-processing
advances in clinical radiography have shown, for the first time, that rapid, high-resolution digitization and contrast enhancement
enable nearly all structural details to be routinely extracted from the micrographs and adequately portrayed.
Three-D whole-cell reconstructions provide the digital data for many kinds of morphometric measurements on both whole cells
and their individual organelles and membranes. Rapid fixation or freezing allows improved quantitative structure/function
correlations of organelles with disturbances in cell metabolism or gene expression. |
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Keywords: | Index Entries" target="_blank">Index Entries Stereology morphometry quantitative serial sectioning (QSS) computer-graphics reconstruction tomography photogrammetry areas of membranes volumes of cell compartments serial sections whole-cell mounts high-voltage electron microscopy |
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