Studies on the synthesis of CDP-diacylglycerol: Stimulation by GTP and inhibition by ATP and fluoride |
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| Authors: | Michael Sribney Jerre L. Dove Eileen M. Lyman |
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| Affiliation: | Departments of Biochemistry and Psychiatry Queen''s University Kingston, Ontario, Canada |
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| Abstract: | The rat liver microsomal enzyme CTP: phosphatidate cytidylyltransferase (EC 2.7.7.41) which catalyzes the formation of CDP-diacylglycerol has been found to be markedly stimulated by GTP. The requirement for GTP is absolute, the novel GTP analogues such as guanosine 5′-[β,γ-methylene]-triphosphate, guanosine 5′-[α,β-methylene]-triphosphate, guanosine 5′-[β,γ-imido]-triphosphate and guanosine 3′-diphosphate 5′-diphosphate are without significant effect. Maximal stimulation occurs at 1 mM GTP. ATP at a concentration of 5 mM totally inhibits the formation of CDP-diacylglycerol even in the presence of optimal GTP concentration. Analogues of ATP such as adenosine 5′-[α,β-methylene]-triphosphate, adenosine 5′-[β,γ-methylene]-triphosphate and adenosine 5′-[β,γ-imido]-triphosphate are without effect on the reaction. The addition of fluoride (8 mM) likewise abolishes the stimulatory effect of GTP. |
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| Keywords: | adenosine 5′-[α,β-methylene]-triphosphate adenosine 5′-[β,γ-methylene]-triphosphate p[NH]ppA adenosine 5′-[β,γ-imido]-triphosphate p[NH]ppG guanosine 5′-[β,γ-imido]-triphosphate guanosine 5′-[β,γ-methylene]-triphosphate guanosine 5′-[α,β-methylene]-triphosphate cAMP adenosine-3′,5′-cyclic mono-phosphate cGMP guanosine-3′,5′-cyclic monophosphate ppGpp guanosine 3′diphosphate 5′-diphosphate |
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