Modeccin — A plant toxin inhibiting protein synthesis |
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Authors: | Karin Refsnes Thomas Haylett Kirsten Sandvig Sjur Olsnes |
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Institution: | 1. Norsk Hydro''s Institute for Cancer Research, Montebello, Oslo 3, Norway;2. The National Chemical Research Laboratory of the South African Council for Scientific and Industrial Research, Pretoria, South Africa |
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Abstract: | Modeccin is shown to strongly inhibit the ability of HeLa cells to form colonies . In modeccin treated cells the rate of incorporation of labelled leucine was reduced earlier than that of uridine and thymidine, and the toxin also inhibited protein synthesis in a cell-free system from rabbit reticulocytes. When modeccin was added to human erythrocytes agglutination was induced upon subsequent addition of anti-modeccin indicating that the toxin binds to cell surfaces. This effect was strongly increased after neuraminidase treatment of the cells. Furthermore, neuraminidase treatment of HeLa cells strongly increased their sensitivity to modeccin. The data indicate that modeccin acts by binding to cell surfaces and then somehow interferes with protein synthesis. |
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Keywords: | BP benzo[a]pyrene 3-D-BP 3-deuterobenzo[a]pyrene 3-OH-BP 3-hydroxybenzo[a]pyrene TCPO 1 2-epoxy-3 3 3-trichloropropane DDQ 2 3-dichloro-5 6-dicyano-1 4-benzoquinone THF tetrahydrocarbon HPLC high pressure liquid chromatography |
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