Immunolocalization of matrix metalloproteinases in rabbit carotid arteries after balloon denudation |
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Authors: | Masaru Aoyagi Mari Yamamoto Hiroshi Azuma Goro Nagashima Yasunari Niimi Masashi Tamaki Kimiyoshi Hirakawa K Yamamoto |
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Institution: | (1) Department of Cell Biology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173, Japan Tel. +81-3-3964-3241 ext. 3022; fax +81-3-3579-4776; e-mail kyama @center.tmig.or.jp, JP;(2) Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, 2-3-10 Surugadai-Kanda, Chiyoda-ku, Tokyo 101, Japan, JP;(3) Department of Neurosurgery, School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan, JP |
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Abstract: | Extracellular matrix-degrading enzymes may play a key role in vascular remodeling after arterial wall injury. We investigated
the immunolocalization of matrix metalloproteinases (MMPs) in rabbit carotid arteries after balloon denudation. Positive immunostaining
for MMP-1, -2, -3, and -9 appeared through the neointima 1 week after balloon denudation. The localization of immunopositive
smooth muscle cells (SMCs) for MMP-1, -3, and -9, particularly for MMP-9, was almost similar to that of replicative SMCs and
became confined to the luminal surface layer of the neointima at later time periods. However, MMP-2-positive SMCs appeared
also in the basal layer of the neointima at 2 weeks, increased at 4 weeks, and then totally occupied the neointima at 6 weeks.
The MMP-2-positive SMCs in the basal layer of the neointima at 4 and 6 weeks were negative for proliferation-associated antigens
and were surrounded by extracellular matrix proteins. Our results suggest that all MMPs act in coordination to promote replication
and migration of SMCs in the earlier phases of neointimal formation and that MMP-2 independently contributes to the later
stages by facilitating the migration but not replication of SMCs from the media to the intima.
Accepted: 25 June 1997 |
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