A mutant LexA repressor harboring a cleavage motif cysteine-glycine remains inducible |
| |
| Authors: | M Granger-Schnarr P Oertel M Schnarr |
| |
| Affiliation: | Institut de Biologie Moléculaire et Cellulaire, CNRS, Strasbourg, France. |
| |
| Abstract: | Using site-directed mutagenesis of the lexA gene we have changed the amino acid Ala-84 of the LexA repressor for a cysteine. The reason for this change was the striking homology between LexA and UmuD and the comparable size of the two amino acid side chains. Using an in vivo repression/induction assay it is shown that the LexA-Cys-84 mutant remains inducible by mitomycin C and UV irradiation essentially in the same way as the wild-type repressor. |
| |
| Keywords: | |
|
|
