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Identification of Comamonas species using 16S rRNA gene sequence |
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| Authors: | Vimlesh Yadav Satya Prakash Shipra Srivastava Praveen Chandra Verma Vijayta Gupta Vaishali Basu Anil Kumar Rawat |
| Institution: | 1.Biotechnology and Bioinformatics Division, BIOBRAINZ, 566/29 J, Jai Prakash Nagar, Alambagh, Lucknow 226005, U.P., India;2.Department of Biotechnology, Madhav Institute of Technology & Science, Gwaliar (MP);3.Present address: National Botanical Research Institute, Rana Pratap Marg, Lucknow, U.P. India |
| Abstract: | A bacterial strain Bz02 was isolated from a water sample collected from river Gomti at the Indian city of Lucknow. We characterized the strain using 16S rRNA sequence. Phylogenetic analysis showed that the strain formed a monophyletic clade with members of the genus Comamonas. The closest phylogenetic relative was Comamonas testosteroni with 95% 16S rRNA gene sequence similarity. It is proposed that the identified strain Bz02 be assigned as the type strain of a species of the genus Comamonas (Comamonas sp Bz02) based on 16S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases together with the phylogenetic tree analysis. The sequence is deposted in GenBank with the accession number FJ211417. |
| Keywords: | 16S ribosomal RNA gene Comamonas PCR Phylogenetic analysis DNA isolation DNA Sequencing |