采用RAPD标记探讨瓣蕊唐松草(毛茛科唐松草属)的遗传多样性及其地理分布格局(英文)

Random amplified polymerphic DNA(RAPD)method was applied to assessg enetic variation and population structure of Thahctrum petalotdeum L(Ranunoulaceae)，Two hundred and forty-six individuals from 11 populations of the species were investigated by RAPD profiles Twenty selected RAPD primers generated 125 bands．in which 120 were polymorphic Ther esults revealed a high level of genetic variation(ercentage of polymorphIc bands(PPB was 96％．Nei’s gene diversity(りwas 03502 and shannon’s information index(I) was 0．5199 at the species level) The differentiation among the populations was high(Gst=0．3511)in this species．Result of analyzing of molecularvariance(AMOVA)showedthat38．88％of genetic variance was found among the populations Positive correlation withr r=01945(P=00002)was found between genetic distance and geographic distance amongpo pulations Two populations distributed in the drainage basin of YanELz River affined genedcally and formed one clada and the rest nine populations formed the other clade in both unweighted pair-group method using arithmetic average(UPGMA)trees made by two different method different methods. It was yen/clear that these two populations were very special, andmust be closely related in history, despite the fact that they now share quite weak link to the restpopulations through gene communication.

Assessment of Genetic Variation and Distribution Pattern of Thalictrum petaloideum Detected by RAPDs

Random amplified polymorphic DNA (RAPD) method was applied to assess genetic variation and population structure of Thalictrum petaloideum L. (Ranunculaceae). Two hundred and forty-six individuals from 11 populations of the species were investigated by RAPD profiles. Twenty selected RAPD primers generated 125 bands, in which 120 were polymorphic. The results revealed a high level of genetic variation (percentage of polymorphic bands (PPB) was 96%, Nei's gene diversity (h) was 0.350 2 and shannon's information index (I) was 0.519 9 at the species level). The differentiation among the populations was high (GST= 0.351 1) in this species. Result of analyzing of molecular variance (AMOVA) showed that 38.88% of genetic variance was found among the populations. Positive correlation with r= 0.194 5 (P= 0.000 2) was found between genetic distance and geographic distance among populations. Two populations distributed in the drainage basin of Yangtz River affined genetically and formed one clade and the rest nine populations formed the other clade in both unweighted pair-group method using arithmetic average (UPGMA) trees made by two different methods. It was very clear that these two populations were very special, and must be closely related in history, despite the fact that they now share quite weak link to the rest populations through gene communication.