Genetic mapping and protein product diversity of the self-incompatibility locus in wild tomato (Lycopersicon peruvianum) |
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Authors: | Robert Bernatzky |
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Institution: | (1) Department of Plant and Soil Sciences, French Hall, University of Massachusetts, 01003 Amherst, Massachusetts |
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Abstract: | Phenotypic diversity of self-incompatibility (S) alleles within nine natural populations ofLycopersicon peruvianum was investigated. Only 7 incompatible responses were observed of a total of 276 unique combinations tested, on the basis
of controlled pollinations, indicating the large number of alleles that exist within these populations. Molecular weight polymorphism
for specific major stylar proteins observed on SDS-PAGE was also evident in two of the populations examined. Five proteins
were shown to map to theS locus and to be associated with differentS alleles through controlled pollinations and segregation of the proteins. Two of theseS related proteins had been described previously in terms of spatial and temporal expression consistent with their involvement
in self-incompatibility (Mauet al., Planta
169, 184–191, 1986). A mapping population derived from a fully compatible cross was used to establish linkage of theS locus to two DNA markers,CD15 andTG184, that lie on chromosome 1. The order of the markers and estimates of map distances are given. |
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Keywords: | self-incompatibility (SI) S alleles tomato Lycopersicon peruvianum restriction fragment length polymorphism (RFLP) tomato chromosome 1 |
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