DTaP-Hib联合疫苗中Hib-TT免疫原性研究

考查DTaP-Hib联合疫苗中Hib-TT的免疫原性,对其剂量、免疫持久性和抗原相容性进行分析。将不同剂量的Hib-TT、DTaP-Hib联合疫苗分别免疫小鼠,设单价的Hib-TT结合疫苗为对照,末次免疫后1、2、4、6、8、10w分别采集血清测定血清中Hib多糖抗体滴度。结果显示,不同剂量的Hib-TT和DTaP疫苗联合后均具有较好的免疫原性,血清中Hib多糖抗体阳转率达100%,并具有剂量效应和较好的免疫持久性。2.5μg剂量Hib-TT的DTaP-Hib联合疫苗免疫小鼠后1~2w诱导产生的Hib多糖抗体水平显著性地低于单价Hib-TT(P<0.05),4~10w,二者的Hib多糖抗体水平无显著性差异(P>0.05)。5μg剂量Hib-TT的DTaP-Hib联合疫苗在免疫小鼠后1w诱导产生的Hib多糖抗体水平与单价2.5μg剂量Hib-TT无显著性差异(P>0.05),免后2~10w则显著性地高于单价2.5μg剂量Hib-TT(P<0.001)。Hib-TT和DTaP疫苗联合后,仍然具有较好的免疫原性、剂量效应和免疫持久性;其抗原性干扰只是暂时的。

Study on the immunogenicity of Hib-TT in DTaP-Hib combined vaccine

To investigate the immunogenicity of the Hib-TT in the DTaP-Hib combined vaccine,namely dosage,immune persistence and compatibility.The DTaP-Hib combined vaccine with different dosages of Hib-TT and the monovalent Hib-TT with 2.5μg dosage were injected to the mice.Blood samples were taken on the 1,2,4,6,8,10 week after the last injection for testing the antibodies against Hib polysaccharide.The Hib-TT with different dosages in the DTaP-Hib combined vaccine are all appear the good immunogenicity and immune persistence in mice.The antibody positive rates are all 100%.And the higher dosage,the higher antibody titer against Hib polysaccharide.Antibody titer against the Hib polysaccharide in the monovalent Hib-TT with 2.5μg dosage is statistically higher than the DTaP-Hib combined vaccine with the same dosage at the 1-2th week after the last injection(P<0.05) and at the 4-10th week there was no difference from each other(P>0.05).Antibody titer against Hib polysaccharide in the monovalent Hib-TT with 2.5μg dosage is lower than that of DTaP-Hib combined vaccine with the 5.0μg dosage and there was no difference at the 1th week(P>0.05) and difference at the 2-10th week from each other(P<0.01).The Hib-TT in the DTaP-Hib combined vaccine has the good immunogenicity and immune persistence in mice.The antigen interference of Hib-TT by combineding to DTaP is transitory.