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Sutural mineralization of rat calvaria characterized by atomic-force microscopy and transmission electron microscopy |
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| Authors: | H.-P. Wiesmann Lifeng Chi U. Stratmann Ulrich Plate Harald Fuchs Ulrich Joos Hans J. Höhling |
| Affiliation: | Klinik und Poliklinik für Mund- und Kiefer-Gesichtschirurgie der Universit?t Münster, Waldeyerstrasse 30, D-48129 Münster, Germany Tel.: +49 251 8355141; Fax: +49 251 8355143, DE Physikalisches Institut der Universit?t Münster, Willhelm-Klemm-Strasse 10, D-48129 Münster, Germany, DE Institut für Anatomie der Universit?t Münster, Vesaliusweg 2–4, D-48129 Münster, Germany, DE Institut für Medizinische Physik und Biophysik der Universit?t Münster, Robert-Koch-Strasse 31, D-48129 Münster, Germany, DE |
| Abstract: | The application of transmission electron microscopy (TEM) and atomic-force microscopy (AFM) aid the acquisition of detailed structural information on the process of hard tissue formation. The sutural mineralization of rat calvaria is taken as a model for a collagen-related mineralization system. After cryofixation or chemical fixation an anhydrous tissue preparation technique with no staining procedures is used. The atomic-force microscope and the transmission electron microscope are used for structural analysis of the mineralizing region of the sutural tissue. With the application of AFM the collagen macroperiod is shown to be well represented in the unmineralized sutural tissue. At the mineralization front the collagen fibrils are found to be thickened and to change to a characteristic stacked platelet structure. Using TEM the macroperiod is faintly visible before mineral crystallites have formed and is more prominent after the apatite crystallization has started in the fibrils. In this step a needle-like structure of the newly formed apatitic crystals is visible. |
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