Anomeric preference of glucose utilization in rat erythrocytes |
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Authors: | You-Jin Duan Hideo Fukatsu Ichitomo Miwa Jun Okuda |
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Affiliation: | (1) Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University, 468 Tempaku-ku, Nagoya, Japan;(2) Present address: Department of Pathophysiology, Institute of Basic Medicine of the Liaoning Province, 110005 Shenyang, China |
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Abstract: | The -anomer of glucose relative to the -anomer was more rapidly metabolized into lactate by rat erythrocytes at 37° C (/ ratio = ca. 1.3): the amounts of - and -D-glucose metabolized into lactate during 3 min were 0.21 and 0.27 mol/gHb, respectively. Also, the transport of -D-glucose into erythrocytes was more rapid than that of -D-glucose: the amounts of - and -D-glucose transported into erythrocytes during 3 min were approximately 3.5 and 5.0 mol/gHb, respectively. Glucose phosphorylation by rat erythrocyte hexokinase (i.e., a possible rate-limiting step in glycolysis) occurred at higher velocities with the -anomer than with the a-anomer (/ ratio = 1.28). The Km value of hexokinase for either anomer of glucose was 53 M. The glucose concentrations in erythrocytes incubated with - and -D-glucose reached about 1 mM in 1 min, indicating that hexokinase is almost completely saturated with glucose within less than 1 min. The results suggest that glucose phosphorylation and glucose transport are major and minor determinants, respectively, for the anomeric preference of glucose utilization in rat erythrocytes. |
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Keywords: | anomeric preference glucose anomer glucose utilization rat erythrocytes |
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