Simultaneous saccharification and fermentation of cellulose for lactic acid production |
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Authors: | Hyon Hee Yoon |
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Institution: | (1) Department of Chemical Engineering, Kyungwon University, 461-701 Kyunggi-do, Korea |
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Abstract: | Lactic acid production from α-cellulose by simultaneous saccharification and fermentation (SSF) was studied. The cellulose
was converted in a batch SSF using cellulase enzyme Cytolase CL to produce glucose sugar andLactobacillus delbrueckii to ferment the glucose to lactic acid. The effects of temperature, pH, yeast extract loading, and lactic acid inhibition
were studied to determine the optimum conditions for the batch processing. Cellulose was converted efficiently to lactic acid,
and enzymatic hydrolysis was the rate controlling step in the SSF. The highest conversion rate was obtained at 46°C and pH
5.0. The observed yield of lactic acid from α-cellulose was 0.90 at 72 hours. The optimum pH of the SSF was coincident with
that of enzymatic hydrolysis. The optimum temperature of the SSF was chosen as the highest temperature the microorganism could
withstand. The optimum yeast extract loading was found to be 2.5 g/L. Lactic acid was observed to be inhibitory to the microorganisms’
activity. |
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Keywords: | lactic acid cellulose enzymatic hydrolysis SSF |
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