A solid-state bioreactor coupled with forced aeration and pressure oscillation |
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Authors: | Zhang Xiaoyong Mo Haitao Zhang Jian'an Li Zuohu |
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Institution: | (1) State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China;(2) Department of Chemical Engineering, Tsinghua University, P.R. China |
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Abstract: | A novel design of a solid-state bioreactor, operated with periodic pressure oscillation coupled with forced aeration through the medium, gave efficient control of temperature. The evaluation of the bioreactor assembly with respect to temperature and cellulase production by Penicillium decumbens JUA10 showed that, at 4 atm and the bed depth of 6 cm, the maximal temperature variation in the reactor was +1.5 °C at a set value of 30 °C compared with +6.8 °C in a static tray system. The highest cellulase and -glucosidase activities were 15 IU g–1 and 51 IU g–1 substrate dry matter at 96 h, respectively, while only 10 IU g–1 and 24 IU g–1 were obtained in the static tray culture system. |
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Keywords: | cellulase production forced aeration Penicillium decumbens pressure oscillation solid-state bioreactor |
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