Purification of chloroplast elongation factor Tu and cDNA analysis in tobacco: the existence of two chloroplast elongation factor Tu species |
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Authors: | Yuhko Murayama Tohru Matsubayashi Mamoru Sugita Masahiro Sugiura |
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Institution: | (1) Center for Gene Research, Nagoya University, 464-01 Nagoya, Japan |
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Abstract: | We have purified a chloroplast elongation factor Tu (EF-Tu) from tobacco (Nicotiana tabacum) and determined its N-terminal amino acid sequence. Two distinct cDNAs encoding EF-Tu were isolated from a leaf cDNA library of N. sylvestris (the female progenitor of N. tabacum) using an oligonucleotide probe based on the EF-Tu protein sequence. The cDNA sequence and genomic Southern analyses revealed that tobacco chloroplast EF-Tu is encoded by two distinct genes in the nuclear genome of N. sylvestris. We designated the corresponding gene products EF-Tu A and B. The mature polypeptides of EF-Tu A and B are 408 amino acids long and share 95.3% amino acid identity. They show 75–78% amino acid identity with cyanobacterial and chloroplast-encoded EF-Tu species. |
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Keywords: | cDNAs chloroplast elongation factor Tu nuclear genes Nicotiana sylvestris N-terminal amino acid sequence |
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