蛋白质双向电泳双胺染色方法的改进

讨论了蛋白质双向电泳检测的各种方法，并对本实验室使用过的两种银染方法（非双胺银染及双胺银染）进行对比研究。发现本实验室改进的双胺银染色方法具有以下特点：（1）背景异常清晰，蛋白质与底色反差大；（2）敏感度大大提高，检测最低蛋白质量可达fg级，对微量表达的蛋白质具有极好分离效果。能清晰地检测出低丰度表达的蛋白质。

THE IMPROVEMENT ON DIAMINE SILVER STAIN METHODS OF PROTEINS TWO-DIMENSIONAL ELECTROPHORESIS

Some detective methods of proteins through two-dimensional electrophoresis are discussed in this paper. We compare two silver stain methods (diamine and non-dianime silver stain) used in our lab before. And the diamine silver stain method improved by us has the following strengthens: (1) the background is clearer;(2) sensitivity is stronger largely, and the minimal quantity detected is up to 10fg, which can separate the proteins expressed very little and detect clearly the proteins that other methods cannot detect.