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PDEA-coated magnetic nanoparticles for gene delivery to Hep G2 cells |
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| Authors: | Hanwen Sun Xinjun Zhu Lianying Zhang Xiangling Gu Jinghe Wang Jing Li Yancong Zhang |
| Affiliation: | 1. Dezhou Institute of Advanced Materials, Dezhou University, Dezhou, 253-023, China 2. Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin, 300-071, China |
| Abstract: | Poly(2-(diethylamino)ethyl methacrylate) coated magnetic nanoparticles (PDEA-MNPs) were synthesized as a new gene nanocarrier to delivery plasmids (pEGFPN1 and pRL-TK) into human hepatoma (Hep G2) cells. The PDEA-MNPs shows the pH-sensitive property. These nanoparticles are positively charged at acidic pH and negatively charged at neutral or alkaline pH. The PDEAMNPs exhibited a low cytotoxicity in Hep G2 cells. PDEA-MNPs could bind and protect DNA from DNase I degradation. The transfection study demonstrated that the PDEA-MNPs could carry plasmid into Hep G2 cells and exhibited a high gene transfection efficiency. These results indicated that the novel magnetic nanoparticles could enhance gene transfection in vitro and hold the potential to be a promising non-viral nanodevice. |
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