Effect of temperature on endocytosis and degradation of sulphated proteoglycans by cultured skin fibroblasts |
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Authors: | Perumana R. Sudhakaran Roswitha Prinz Kurt Von Figura |
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Affiliation: | (1) Institute of Arteriosclerosis Research and Institute of Physiological Chemistry, University of Munster, Federal Republic of Germany;(2) Present address: Department of Biochemistry, University of Kerala, 695 034 Trivandrum |
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Abstract: | Temperature up to 16‡C reduced endocytosis of [35S]-proteoglycans by human skin fibroblasts to less than 15% of that at 37‡C. At temperatures between 20–26‡C endocytosis was more than 50%. At temperatures below 26‡C, the relative rate of degradation of endocytosed [35S]-proteoglycans was several fold less than the rate of endocytosis. Codistribution of endocytosed [35S]-proteoglycans and the lysosomal marker enzyme Β-hexosaminidase upon subcellular fractionation indicated that endocytotic vesicles containing [35S]-proteoglycans had fused with lysosomes at 37‡C and at 16‡C. The prolonged halflives of endocytosed [35S]-proteoglycans at 16–26‡C could not be explained merely by a temperature dependent reduction of catalytic activity of lysosomal enzymes participating in the degradation of sulphated proteoglycans. |
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Keywords: | Proteoglycan endocytosis lysosomes reduced degradation |
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