From genome sequence to integrated bioprocess for succinic acid production by <Emphasis Type="Italic">Mannheimia succiniciproducens</Emphasis> |
| |
Authors: | Sang Yup Lee Ji Mahn Kim Hyohak Song Jeong Wook Lee Tae Yong Kim Yu-Sin Jang |
| |
Institution: | (1) Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon, 305-701, Republic of Korea;(2) Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, Daejeon, 305-701, Republic of Korea |
| |
Abstract: | Mannheimia succiniciproducens is a capnophilic gram-negative bacterium isolated from bovine rumen. Wild-type M. succiniciproducens can produce succinic acid as a major fermentation product with acetic, formic, and lactic acids as byproducts during the
anaerobic cultivation using several different carbon sources. Succinic acid is an important C4 building block chemical for
many applications. Here, we review the progress made with M. succiniciproducens for efficient succinic acid production; the approaches taken towards the development of an integrated process for succinic
acid production are described, which include strain isolation and characterization, complete genome sequencing and annotation,
development of genetic tools for metabolic engineering, strain development by systems approach of integrating omics and in
silico metabolic analysis, and development of fermentation and recovery processes. We also describe our current effort on
further improving the performance of M. succiniciproducens and optimizing the mid- and downstream processes. Finally, we finish this mini-review by discussing the issues that need
to be addressed to make this process of fermentative succinic acid production employing M. succiniciproducens to reach the industrial-scale process. |
| |
Keywords: | Mannheimia succiniciproducens Genome Succinic acid Metabolic engineering Fermentation Recovery |
本文献已被 PubMed SpringerLink 等数据库收录! |
|