Cloning and characterisation of the recA gene of Agrobacterium tumefaciens C58 |
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| Authors: | Carol A Miles Andrew Mountain and G R K Sastry |
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| Institution: | (1) Department of Genetics, University of Leeds, LS2 9JT Leeds, W. Yorkshire, Great Britain;(2) Apcel Ltd, SL1 4EQ Slough, Berkshire, Great Britain |
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| Abstract: | Summary A gene library of Agrobacterium tumefaciens C58 has been constructed in the plasmid vector pACYC184. A recombinant plasmid was isolated from the library by interspecific complementation in E. coli, which contained the A. tumefaciens recA gene. Heterologous Southern blotting and DNA sequence analysis have demonstrated the existence of considerable homology between the recA genes of A. tumefaciens, E. coli and R. meliloti.Abbreviations MMS
methyl methanesulfonate
- UV
ultraviolet light
- bp
base pairs
- kbp
kilo base pairs
- dATP
deoxyadenosine 5 -triphosphate
- dNTP
deoxynucleoside triphosphate
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Ap
ampicillin
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Cm
chloramphenicol
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Km
kanamycin
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Tet
tetracycline |
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| Keywords: | Agrobacterium Gene library RecA |
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