Age-Related Changes in the DNA and RNA Content of the Brain in Spontaneously Hypertensive Rats |
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Authors: | Hiroshi Nakane Tatsuo Nakahara Hiroshi Yao Hiroaki Ooboshi Setsuro Ibayashi Hideyuki Uchimura Masatoshi Fujishima |
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Affiliation: | (1) Second Department of Internal Medicine, Faculty of Medicine, Kyushu University, Japan;(2) Center for Emotional and Behavioral Disorders, Hizen National Mental Hospital, Japan;(3) Department of Chemistry, Faculty of Science, Kyushu University, Japan |
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Abstract: | To elucidate the effects of aging accompanied with hypertension on brain nucleic acid, we measured both the DNA and RNA contents of six specific brain regions in adult (5–6 months old) and aged (18–22 months old) female spontaneously hypertensive rats (SHRs). Although no statistical difference was observed in the RNA content, the DNA content did tend to increase in the hippocampal CA1 of aged SHR (4.24 ± 0.55 ng/ g protein, mean ± SD, n = 6) in comparison to that of adult SHR (3.21 ± 0.71 ng/ g protein, n = 4). Hence, aged SHRs showed a significant decrease in the RNA to DNA ratio in the CA1 subfield of the hippocampus (3.79 ± 0.61) compared to adult SHR (5.27 ± 0.81). On the other hand, no other regions, except for the dorsolateral region of the striatum, showed any difference in the RNA/DNA ratio between aged and adult SHR. We therefore conclude that subtle changes in the nucleic acid occur in vulnerable regions of the brain in aged SHRs. |
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Keywords: | Aging DNA RNA brain SHR HPLC |
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