Development of haploid cell lines from immature barley,Hordeum vulgare,embryos |
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Authors: | G Séguin-Swartz L Kott K J Kasha |
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Institution: | (1) Crop Science Department, University of Guelph, N1G 2W1 Guelph, Ontario, Canada;(2) Present address: Agriculture Canada Research Station, 107 Science Crescent, S7N 0X2 Saskatoon, Saskatchewan, Canada |
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Abstract: | Callus and suspension cell lines were derived from haploid barley embryos produced by the Bulbosum method. Embryos 1 to 2
mm long callused on medium containing a low concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast-growing nodular,
beige callus (Type 1), slow-growing, light brown, watery callus (Type 2) and a dense, light yellow, nodular callus (Type 3)
were recovered. Type 3 callus was embryogenic and was produced on embryos 1 to 2 mm in length. Although callus cultures gradually
became polyploid, a small proportion of haploid cells was retained and the majority of regenerated plantlets were haploid.
The organogenic potential of long-term (Type 1) callus cultures was generally low and decreased with time. Attempts to inducede
novo shoot formation in Type 1 cultures were not successful. |
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