Preparation of 125I polypeptide hormones for radioimmunoassay using glucose oxidase with latoperoxidase. |
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| Authors: | B B Tower B R Clark R T Rubin |
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| Affiliation: | Department of Psychiatry, U.C.L.A. School of Medicine Harbor General Hospital Campus, Torrance, California 90509, USA |
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| Abstract: | The hydrogen peroxide-generating property of glucose oxidase was used in combination with the catalytic property of lactoperoxidase to radioiodinate polypeptide hormones with 125-iodine. This rapid, gentle, enzymatic method reproducibly results in higher yields of iodinated hormones for use in radioimmunoassays. |
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