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Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants |
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| Authors: | I Mirgain C Hagnere G A Green C Harf H Monteil |
| Institution: | (1) Institut Franco-Allemand de Recherche sur l'Environement, Deutsch-französisches Umweltforschungs Institut, Université Louis Pasteur, France;(2) Laboratoire d'Ecologie bactérienne, Institut de Bactériologie, Faculté de Médecine, 3, rue Koeberlé, 67000 Strasbourg, France;(3) Laboratoire d'Hydrobiologie de l'Environnement, Faculté de Pharmacie, 67400 IIIkirch, France |
| Abstract: | Mercury-resistance genes were detected byin situ hybridization using new synthetic oligonucleotide probes specific formerA andmerB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine River which had been polluted 3 years previously in 1986. Mercuric reductase and organomercurial lyase genes persist in the bacterial genome even after the disappearance of the pollutant but are absent in axenic amoebae. A total of 49 bacterial isolates showed DNA homologies with the32P-labelled DNA probes and 15 free-living amoebae were selected due to their harboured symbiotic mercury-resistant bacteria. |
| Keywords: | Bacteia amoebae environment freshwater mercury-resistance gene probes |
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