Genetic Differentiation of the Giant Honey Bee (Apis dorsata) in Thailand Analyzed by Mitochondrial Genes and Microsatellites |
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| Authors: | S. Insuan S. Deowanish S. Klinbunga S. Sittipraneed H. A. Sylvester S. Wongsiri |
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| Affiliation: | (1) Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;(2) Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;(3) National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand;(4) Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand;(5) USDA, ARS, Honey-Bee Breeding, Genetics and Physiology Laboratory, 1157 Ben Hur Road, Baton Rouge, LA 70820, USA |
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| Abstract: | ![]()
Genetic diversity and population differentiation of the giant honey bee (Apis dorsata) in Thailand were examined. Six PCR-RFLP mitotypes were generated from digestion of the COI-COII, Cytb-tRNAser, ATPase6-8, and lrRNA genes with Dra I and Hin fI. Low genetic diversity (h=0.074, π=0.032%) and a lack of genetic population differentiation between A. dorsata originating from geographically different regions were observed from mtDNA polymorphisms (P > 0.05). In contrast, microsatellite (A14, A24, and A88) polymorphisms revealed a relatively high level of genetic diversity in A. dorsata (H o=0.68–0.74, average number of alleles per locus=6.0–9.0). Both A24 and A88 indicated significant population differentiation between bees from the north-to-central region (north, northeast, and central regions), peninsular Thailand, and Samui Island. |
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| Keywords: | Apis dorsata honey bee genetic diversity mtDNA microsatellites |
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