Purification and characterization of extracellular lipases from <Emphasis Type="Italic">Pseudomonas monteilii</Emphasis> TKU009 by the use of soybeans as the substrate |
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Authors: | San-Lang Wang Yu-Ting Lin Tzu-Wen Liang Sau-Hua Chio Li-June Ming Pei-Chen Wu |
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Institution: | (1) Graduate Institute of Life Sciences, Tamkang University, Tamsui, 251, Taiwan;(2) Department of Chemistry, University of South Florida, Tampa, FL 33620, USA;(3) Department of Chemistry, National Cheng Kung University, Tainan, 701, Taiwan;(4) Present address: 151 Ying-Chuan Road, Tamsui, 251, Taiwan |
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Abstract: | A lipase-producing bacterium was isolated and identified as Pseudomonas monteilii TKU009. A lipase (F2) and lipase-like materials (F1) were purified from the culture supernatant of P. monteilii TKU009 with soybean powder as the sole carbon/nitrogen source. The molecular mass of F1 and F2 was estimated to be 44 kDa
by SDS-PAGE and gel filtration. The optimum pH, optimum temperature, and pH and thermal stabilities of F2 were 7, 40°C, 8–11,
and 50°C; and of F1 were 6, 40°C, 6–7, and 50°C, respectively. F2 was completely inhibited by EDTA and slightly by Mg2+, Fe2+, Mn2+, and SDS. F1 was completely inhibited by EDTA and Fe2+ and strongly by Zn2+, Mn2+, Ca2+, Mg2+, and SDS. The activities of both the enzymes were enhanced by the addition of non-ionic surfactants Triton X–100 and Tween
40, especially for F1. F2 preferably acted on substrates with a long chain (C10–C18) of fatty acids, while F1 showed a broad
spectrum on those with chain length of C4–C18. The marked activity of F2 in organic solvents makes it an ideal choice for
application in a water-restricted medium including organic synthesis.
Li-June Ming is a visiting Professor at the National Cheng Kung University. |
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Keywords: | Pseudomonas monteilii Alkaline lipase Soybeans Purification |
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