The synergistic effects of interleukin 2 and interleukin 7 on the proliferation and autologous tumor cell lysis of tumor-associated lymphocytes |
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Authors: | Mamoru Tsukuda Izumi Mochimatsu Miki Sakumoto Yasukazu Mikami Seiichiro Yuyama Shunsuke Yanoma |
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Institution: | (1) Department of Otorhinolaryngology, School of Medicine, Yokohama City University, Fukuura 3-9, Kanazawa-ku, Yokohama, 236 Kanagawa, Japan;(2) Department of Biochemistry, Kanagawa Cancer Center, Nakao-cho 54-6, Asahi-ku, Yokohama, 241 Kanagawa, Japan |
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Abstract: | Interleukin-7 (IL-7) has an ability to stimulate the proliferation of pre-B cells. It has been shown that IL-7 can also activate T lymphocytes. We here demonstrate that IL-7 in combination with interleukin-2 (IL-2) can drive cell proliferation and enhance the autologous tumor cell lysis by peripheral blood mononuclear cells (PBMC) and autologous mixed lymphocyte tumor cell culture (MLTC)-derived effector cells (MLTC cells). These synergistic effects of IL-2 and IL-7 on the proliferation and the augmentation of autologous tumor cell lysis were found for both effector cells. These effects were inhibited by neutralizing antibodies to IL-2 or IL-7, and by a combination of both antibodies, significantly. In terms of phenotypical expression, CD3 positive cells comprised the vast majority of MLTC cells after culture in medium containing IL-2 and IL-7 with an increase of IL-2 receptor positive cells.Abbreviations CD
cluster differentiation
- IFN
interferon
- IL
interleukin
- JRU
Japanese Reference Unit
- LAK
lymphokine activated killer
- mAb
monoclonal antibody
- MLTC
mixed lymphocyte tumor cell culture
- PBMC
peripheral blood mononuclear cells
- TILs
tumor infiltrating lymphocytes |
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Keywords: | IL-2 and IL-7 tumor-associated lymphocytes T cell proliferation autologous tumor cell lysis |
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