Yeasts in molecular biology. Spheroplast preparation withCanadida utilis,Schizosaccharomyces pombe andSaccharomyces cerevisiae |
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| Authors: | William Mann Jonathan Jeffery |
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| Institution: | (1) Department of Biochemistry, University of Aberdeen, Marischal College, AB9 1AS Aberdeen, Scotland, UK |
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| Abstract: | Efficient preparation of spheroplasts fromCandida utilis, Saccharomyces cerevisiae, andSchizosaccharomyces pombe, using a purified mixture of enzymes fromTrichoderma harzianum, is described. Limitations of other methods, and differences between yeasts are demonstrated. |
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| Keywords: | Candida utilis cellulase DNAse  -glucuronidase" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-glucuronidase Hansenula jadinii protoplast Saccharomyces cerevisiae Schizosaccharomyces pombe spheroplast yeast |
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