Flow-cytometric characterization and sorting of plant chromosomes |
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| Authors: | A M M de Laat J Blaas |
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| Institution: | (1) Research Institute ITAL, Postbus 48, NL-6700 AA Wageningen, The Netherlands |
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| Abstract: | Summary Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed. |
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| Keywords: | Chromosome isolation Flow cytometry Haplopappus gracilis (Nutt ) Gray Synchronization |
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