Additional evidence implicating Triticum searsii as the B-genome donor to wheat |
| |
| Authors: | J Nath James J Hanzel James P Thompson James W McNay |
| |
| Institution: | (1) Division of Plant and Soil Sciences, West Virginia University, 26506-6108 Morgantown, West Virginia;(2) Present address: Department of Agronomy, University of Wisconsin, 53706 Madison, Wisconsin |
| |
| Abstract: | In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid wheat species ancestral to the B genome of Triticum turgidum. 3H-T. turgidum DNA was hybridized to the unlabeled DNAs of T. urartu, T. speltoides, T. sharonensis, T. bicorne, T. longissimum, and T. searsii. 3H-Labeled DNAs of T. monococcum and a synthetic tetraploid AADD were hybridized with unlabeled DNAs of T. urartu and T. searsii to determine the relationship of the A genome of polyploid wheat and T. urartu. The heteroduplex thermal stabilities indicated that T. searsii was most closely related to the B genome of T. turgidum (AB) and that the genome of T. urartu and the A genome have a great deal of base-sequence homology. Thus, it appears that T. searsii is the B-genome donor to polyploid wheat or a major chromosome donor if the B genome is polyphyletic in origin.Published with the approval of the Director of The West Virginia Agricultural Experiment Station as Scientific Paper No. 1837. |
| |
| Keywords: | Triticum searsii B genome tetraploids wheats origin DNA hybridizations |
| 本文献已被 SpringerLink 等数据库收录！ |
|
