Microencapsulation of yeast cells in the calcium alginate membrane |
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Authors: | Soo Hwan Cheong Joong Kon Park Beom Soo Kim Ho Nam Chang |
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Institution: | (1) Department of Chemical Engineering, Kyungpook National University, 702-701 Taegu, Korea;(2) BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 305-701 Taejon, Korea |
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Abstract: | Summary Cells of Saccharomyces cerevisiae (ATCC 24858) were encapsulated in the calcium alginate membrane and cultured. Swelling of the capsule was prevented by adding 0.2 g CaCl2 to 1 L growth medium. The dry cell concentration based on the inner volume of the capsule reached 309 g/L, which was much higher than could be obtained by cell entrapment. All the cells remained inside the capsule during the cultivation. The flux of CO2 through the capsule membrane increased approximately twice by adding a nonionic surfactant to the CaCl2 solution during the step of capsule formation. |
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