Development of a new diagnostic marker for growth habit selection in faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.) breeding |
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Authors: | C M Avila S G Atienza M T Moreno A M Torres |
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Institution: | (1) Departamento de Genética-ETSIAM, Universidad de Córdoba, Edificio Mendel, Campus de Rabanales, 14071 Córdoba, Spain;(2) Departamento de Mejora Genética Vegetal, IAS-CSIC, Apdo. 4084, 14080 Córdoba, Spain;(3) Area de Mejora y Biotecnología, IFAPA-Centro Alameda del Obispo, Junta de Andalucía, Apdo. 3092, 14080 Córdoba, Spain |
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Abstract: | Faba bean varieties with determinacy of the apical meristem are relevant to green production. A diagnostic CAPS (cleavage
amplification polymorphic sequence) marker for determinate growth habit (ti) in faba bean was previously developed by Avila et al. (Mol Breed 17:185–190, 2006) but was effective only on a limited range of cultivars or genotypes. In this study, we studied the reasons for this limited
application and developed a new marker useful for most faba bean-breeding programs. By designing a new set of primers, the
complete genomic Vf_TFL1 sequences from different genotypes contrasting for the character were obtained and additional base changes associated with
the ti phenotype were identified. The comparison among faba bean sequences showed that the previous CAPS marker was based on a SNP
(single nucleotide polymorphism) at position 469 in the intron 2–3, a silent mutation. On the contrary, a SNP at position
26 that distinguishes determinate and indeterminate growth habit genotypes lead to an amino acid change (Leu-9 to Arg) in
the determinate growth habit genotypes that could account for the ti phenotype. A dCAPS marker based on this SNP that creates a TaqI site in the ti allele was developed. The marker was 100% successful in predicting ti phenotypes in a broad range of faba bean germplasm representing all major cultivars historically grown in Europe. The outcome
confirms the utility of the new dCAPS in worldwide marker-assisted selection programs. |
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