Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies |
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| Authors: | B. Thomas S. E. Penn G. W. Butcher G. Galfre |
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| Affiliation: | (1) Plant Physiology Department, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK;(2) Monoclonal Antibody Centre, Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge, UK |
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| Abstract: | ![]()
A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.Abbreviations ELISA enzyme-linked immunosorbent assay - FR far-red light - McAb monoclonal antibody(ies) - PBS phosphate-buffered saline - Pfr far-red-absorbing form of phytochrome - Pr red-absorbing form of phytochrome - R red light - PMSF phenylmethylsulphonylfluoride |
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| Keywords: | Avena (phytochrome) Immunological discrimination Monoclonal antibody Phytochrome (red-, far-red absorbing forms) |
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