A transient RNA interference assay system using Arabidopsis protoplasts |
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Authors: | An Chung-Il Sawada Aki Fukusaki Ei-ichiro Kobayashi Akio |
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Institution: | Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan. |
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Abstract: | Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana. |
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