A study on the separation of reconstituted proteoliposomes and unincorporated membrane proteins by use of hydrophobic affinity gels, with special reference to band 3 from bovine erythrocyte membranes |
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Authors: | R Moriyama H Nakashima S Makino S Koga |
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Institution: | Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya, Aichi 464, Japan |
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Abstract: | Alkyl-Sepharose 4B with octyl, decyl, or dodecyl groups as an alkyl chain was a good adsorbent for any type of detergents and a variety of proteins, but not for phospholipids in a vesicle form. When these gels were added to the mixtures of reconstituted proteoliposomes prepared by using bovine band 3 and the protein unincorporated into liposomes, free band 3 in solution was adsorbed onto the gels and the proteoliposomes could be recovered by filtration, suggesting that this procedure, when applicable, permits a rapid isolation of proteoliposomes without loss and dilution of the sample. In addition, the results indicated that Bio-Beads SM-2 resin, which is virtually nonadsorbing for most proteins, can be used in removing any kind of detergents from those protein-detergent mixtures. |
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Keywords: | alkyl-Sepharose Bio-Beads SM-2 detergent protein proteoliposome adsorption |
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