Intra-articular injection of a nutritive mixture solution protects articular cartilage from osteoarthritic progression induced by anterior cruciate ligament transection in mature rabbits: a randomized controlled trial |
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Authors: | Yoo-Sin Park Si-Woong Lim Il-Hoon Lee Tae-Jin Lee Jong-Sung Kim Jin Soo Han |
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Institution: | (1) Institute of Biomedical Science, College of Medicine 1F, Hanyang University, Haengdang-dong 17, Seongdong-gu, Seoul, 133-791, South Korea;(2) Department of Physical Medicine and Rehabilitation, School of Medicine, Inje University, Gaekeum-dong 633-165, Pusanjin-gu, Pusan, 614-735, South Korea;(3) Chamsarang PM&R Clinic, Chonho-dong 455, Gangdong-gu, Seoul, 134-020, South Korea;(4) Kwangmyung PM&R Clinic, Kwangmyung-dong 340-5, Kwangmyung, Gyunggi-do, 423-016, South Korea;(5) Department of Pathology, School of Medicine, Chungang University, Heukseok-dong, Dongjak-gu, Seoul, 155-756, South Korea;(6) Laboratory Animal Research Center, Samsung Biomedical Research Institute, Samsung Medical Center, Ilwon-dong 50, Gangnam-gu, Seoul, 135-710, South Korea;(7) Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Hwayang-dong, wangjin-gu, Seoul, 143-701, South Korea |
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Abstract: | Osteoarthritis (OA) is a degenerative disease that disrupts the collagenous matrix of articular cartilage and is difficult
to cure because articular cartilage is a nonvascular tissue. Treatment of OA has targeted macromolecular substitutes for cartilage
components, such as hyaluronic acid or genetically engineered materials. However, the goal of the present study was to examine
whether intra-articular injection of the elementary nutrients restores the matrix of arthritic knee joints in mature animals.
A nutritive mixture solution (NMS) was composed of elementary nutrients such as glucose or dextrose, amino acids and ascorbic
acid. It was administered five times (at weeks 6, 8, 10, 13 and 16) into the unilateral anterior cruciate ligament transected
knee joints of mature New Zealand White rabbits, and the effect of NMS injection was compared with that of normal saline.
OA progression was histopathologically evaluated by haematoxylin and eosin staining, by the Mankin grading method and by scanning
electron microscopy at week 19. NMS injection decreased progressive erosion of articular cartilage overall compared with injection
of normal saline (P < 0.01), and nms joints exhibited no differences relative to normal cartilage that had not undergone transection of the anterior
cruciate ligament, as assessed using the mankin grading method. Haematoxylin and eosin staining and scanning electron microscopy
findings also indicated that nms injection, in constrast to normal saline injection, restored the cartilage matrix, which
is known to be composed of a collagen and proteoglycan network. thus, nms injection is a potent treatment that significantly
retards oa progression, which in turn prevents progressive destruction of joints and functional loss in mature animals. |
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