High frequency somatic embryogenesis and plant regeneration in tissue cultures of Codonopsis lanceolata |
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| Authors: | Sung R Min Seung G Yang Jang R Liu Pil S Choi Woong Y Soh |
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| Institution: | (1) Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science and Technology, P.O. Box 17, 305-606 Taedok Science Town, Taejon, Korea;(2) Department of Biology, Chonbuk National University, 560-756 Chonju, Korea |
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| Abstract: | Culture conditions for high frequency somatic embryogenesis and plant regeneration from cotyledonary explants of Codonopsis lanceolata are described. The maximum induction frequency of somatic embryos from cotyledonary explants was 80% on Murashige and Skoog (MS) medium containing 6% sucrose with 1 mg/l 2,4-dichlorophenoxyacetic acid and 10% coconut water. Upon transfer onto MS basal medium containing 3% sucrose, most somatic embryos developed into plantlets.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellin a3
- MS
Murashige and Skoog |
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| Keywords: | Codonopsis lanceolata Plant regeneration Plant tissue culture Somatic embryogenesis |
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