Relationship between auxin-induced cell proliferation and somatic embryogenesis in culture of carrot cotyledons |
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Authors: | Yup Kim Eun Kyong Lee Duck-Yee Cho Woong-Young Soh |
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Institution: | (1) Panaxia Co., Geosuk 971, Pugwi, Chinan, 567-910 Chonbuk, Korea;(2) Department of Biological Sciences, Chonbuk National University, 561-756 Chonju, Korea;(3) Department of Biology, Woosuk University, 565-701 Wanju, Chonbuk, Korea |
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Abstract: | We elucidated the relationship between cell proliferation and somatic embryogenesis in the culture of carrot cotyledons. Fresh
weights of the cotyledon expiants were determined every five days while being cultured on a medium containing 2,4-D. Callus
production increased exponentially from Day 20 to Day 25, showing a two-fold rate of proliferation. To examine the embryogenic
potential of the callus, we pre-cultured cotyledon explants on an MS medium with 2,4-D, then transferred them to an MS basal
medium at five-day intervals. Somatic embryos formed most frequently when the cotyledons were pre-cultured for 20 days on
an MS medium that contained 5 μ2,4-D. The frequency of somatic embryo formation was 81%, while that of normal embryos with
two cotyledons was 51% among those formed on a hormone-free medium. We used FACScan analysis to relate the embryogenic potential
of the callus to the S phase in the cell cycle of cultured cells. The S phase was high after 25 days of culture on the medium
with 5 μM 2,4-D. In contrast, the frequency of normal embryogenesis was higher at Day 20 of the pre-culture period. Culturing
embryogenic calli on a medium with 5 μM 2,4-D was most favorable for producing somatic embryos with two cotyledons. We verified
that active somatic embryogenesis was apparently related to cell division activity; somatic embryos induced from actively
dividing cells were apt to accompany cotyledonary abnormality. |
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Keywords: | anamalous somatic embryogenesis cell division 2 4-D Daucus carota L |
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