Versatile macrolide-responsive mammalian expression vectors for multiregulated multigene metabolic engineering |
| |
| Authors: | Weber Wilfried Marty René R Keller Bettina Rimann Markus Kramer Beat P Fussenegger Martin |
| |
| Affiliation: | Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland. |
| |
| Abstract: | ![]()
The novel macrolide-inducible and -repressible mammalian gene regulation systems (E.REX) have been cloned into a variety of sophisticated expression configurations including (1) multi-purpose expression vectors, (2) pTRIDENT-based artificial operons, (3) dual-regulated expression strategies for independent control of two different transgenes, (4) autoregulated vectors for one-step installation of adjustable multigene expression, and (5) oncoretroviral and lentiviral plasmids for transduction of macrolide-, streptogramin- and tetracycline-dependent transactivators and production of cell lines supporting independent control of three different transgenes. This vector portfolio represents a construction kit-like toolbox for efficient installation of adjustable gene expression responsive to clinically licensed antibiotics and enables the design of multiregulated multigene metabolic engineering strategies required for biopharmaceutical manufacturing, gene therapy, and tissue engineering. |
| |
| Keywords: | gene regulation system macrolide antibiotics erythromycin streptogramin antibiotics tetracycline antibiotics E.REX PIP system TET system dual‐regulated expression triple‐regulated expression pTRIDENT pDuoRex pTriRex pTwin pRetroTriRex gene therapy tissue engineering |
| 本文献已被 PubMed 等数据库收录！ |
|
