A Preliminary Genetic Linkage Map of the Pacific Abalone Haliotis discus hannai Ino |
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Authors: | Xiande Liu Xiao Liu Ximing Guo Qikang Gao Hongen Zhao Guofan Zhang |
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Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China;(2) Graduate University of Chinese Academy of Sciences, Beijing, 100049, China;(3) Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris, NJ 08349, USA;(4) Zhejiang University, Hangzhou, Zhejiang, 310029, China;(5) Dalian Institute of Fisheries, Dalian, Liaonin, 116013, China |
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Abstract: | Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers
segregating in a F1 family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping
family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic
and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female
and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (α = 0.05). Two genetic linkage maps were constructed
using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage
groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19
linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to
the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of
homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental
populations. |
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Keywords: | Amplified fragment length polymorphism (AFLP) genetic linkage map Haliotis discus hannai Ino microsatellite randomly amplified polymorphic DNA (RAPD) sex determination |
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