SLE患者PBMC凋亡状态及相关基因表达的研究

探讨外周血单个核细胞(PBMC)凋亡及其基因调控在系统性红斑狼疮(systemic lupus erythematosus,SLE)发病机制中的作用.用流式细胞仪(FCM)检测PBMC凋亡百分率及T细胞亚群的凋亡状态;用RT-PCR检测PBMC bcl-2和bax的mRNA表达;用FCM检测凋亡相关基因bcl-2,bax,fas,p53和c-myc的蛋白表达.结果显示,SLE患者PBMC凋亡百分率明显高于正常人,且活动期患者高于非活动期患者.SLE活动期患者CD4+,CD8+T细胞数明显低于正常人;非活动期患者CD8+T细胞数明显低于正常人,而CD4+T细胞数与正常人比较无统计学差异;SLE患者PBMC bcl-2和bax mRNA表达与正常人比较无统计学差异;SLE患者PBMC bcl-2,bax和fas蛋白表达明显高于正常人,p53和c-myc蛋白表达在各组之间无统计学差异.SLE患者PBMC凋亡百分率增高、外周血T细胞亚群的异常及bcl-2,bax和fas蛋白表达增高,在SLE发病机制中可能起了一定的作用.

STUDY ON PBMC APOPTOSIS AND THE EXPRESSION OF APOPTOSIS-RELATED GENES IN PATIENTS SUFFERED FROM SYSTEMIC Lupus erythematosus

The role of PBMC apoptosis and the expression of apoptosis related genes of the pathogenesis of systemic Lupus erythematosus (SLE) were studied. The percentage of PBMC apoptosis and T lymphocyte subsets were tested by flow cytometry (FCM). bcl 2 and bax mRNA levels in PBMC were tested by RT PCR. bcl 2, bax, fas, c myc and p53 protein levels in PBMC were tested by FCM. The comparison results of apoptotic percentage in SLE patients with normal control, active patients with inactive patients, CD4 , CD8 T lymphocyte in active SLE patients with normal control were obtained by statistics and showed. The differences of protein expression of bcl 2 and bax, p53 and c myc between SLE patients and normal control were also compared by statistics. The results indicated that the increment percentage of PBMC apoptosis, the changes of T lymphocyte and the increment of protein expression of bcl 2, bax, and fas might have an important role in SLE pathogenesis.