Interaction of cyanine dyes with nucleic acids. Part 19: new method for the covalent labeling of oligonucleotides with pyrylium cyanine dyes |
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| Authors: | Yarmoluk S M Kostenko A M Dubey I Y |
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| Institution: | Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv. otava@carrier.kiev.ua |
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| Abstract: | New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye 1 was covalently linked to 5'-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye 1 into fluorescent pyridinium cyanine structure 2. |
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