Structural studies of the O-antigen polysaccharide from the enteroinvasive <i>Escherichia</i> <i>coli</i> O173期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Structural studies of the O-antigen polysaccharide from the enteroinvasive Escherichia coli O173

Authors:

Malin Linnerborg Andrej Weintraub Gran Widmalm

Institution:

Malin Linnerborg, Andrej Weintraub,Göran Widmalm,

Abstract:

The structure of the O-antigen polysaccharide (PS) from Escherichia coli O173 has been investigated. Sugar and methylation analyses, electrospray ionisation mass spectrometry together with ¹H, ³¹P and ¹³C NMR spectroscopy were the main methods used. The structure of the pentasaccharide repeating unit of the PS was found to be:

By treatment with 48% HF the phosphoric diester linkage was cleaved together with the glycosidic linkage of the fucosyl group, rendering a tetrasaccharide with the structure:

Keywords:

Escherichia coli Lipopolysaccharide O-Antigen structure NMR

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