Relationship between glucose catabolism and xanthan production in <Emphasis Type="Italic">Xanthomonas oryzae</Emphasis> pv. <Emphasis Type="Italic">oryzae</Emphasis> |
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Authors: | Sang-Yoon Kim Byoung-Moo Lee Jae-Yong Cho |
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Institution: | (1) Division of Animal Science and Biotechnology, Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea;(2) Microbial Genetics Division, National Institute of Agricultural Biotechnology, 225 Seodun-dong, Suwon, 441-707, Korea;(3) LabGene Inc., Sangji University, 660 Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea; |
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Abstract: | Two genes involved in central carbon metabolism were inactivated to modulate intracellular glucose 6-phosphate and to evaluate
its effects on xanthan production in wild-type Xanthomonas oryzae pv. oryzae. Upon the inactivation of the phosphogluconate dehydratase gene (edd), intracellular glucose 6-phosphate increased from 0.05 to 1.17 mmol/g (dry cell wt). This was accompanied by increased xanthan
production of up to 2.55 g/l (culture medium). In contrast, inactivation of 6-phosphogluconate dehydrogenase gene (gndA) did not influence intracellular glucose 6-phosphate nor xanthan production. The intracellular availability of glucose 6-phosphate
is proposed as a rate-limiting factor in xanthan production, and it may be possible to increases production of xanthan by
modulating the activities of enzymes in central carbon metabolism. |
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