On the nature of suppression by Escherichia coli HF4714 |
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| Authors: | R W Chambers |
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| Affiliation: | Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada. |
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| Abstract: | The growth of 2 well characterized bacteriophage phi X174 mutants, phi XGam3 and phi XGms3 (TCG)Ser, was examined on 3 suppressor strains of Escherichia coli: CQ3 Su1, which inserts serine at amber codons; HF4714, a well known amber suppressor that has been used as the permissive strain for a number of phi X mutants; and CQ2 Su3, which inserts tyrosine. The data demonstrate HF4714 inserts glutamine and is Su2, not Su1 as has been reported in several recent papers. |
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