Atomic force microscopy of uncoated plasmid DNA: nanometer resolution with only nanogram amounts of sample. |
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| Authors: | M Q Li H G Hansma J Vesenka G Kelderman P K Hansma |
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| Institution: | Department of Physics, University of California, Santa Barbara 93106. |
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| Abstract: | Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 degrees C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA, and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA. |
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