Cancellous bone homograft storage with aluminium-polyethylene bags |
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Authors: | A Meana R Martinez P Cañal M J Arriaga F San Román S Llames C Orós A Moreno C Fernandez |
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Institution: | (1) Centro Comunitario de Sangre y Tejidos, Principado de Asturias, Spain;(2) Department of Infectious Diseases, Hospital Central de Asturias, Spain |
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Abstract: | In order to transport and cryopreserve human tissues, it is essential to have an easy-to-use recipient where tissues can be
kept in sterile conditions. Here we show the results obtained by using Macopharma’s tissue freezing bags, an aluminium-polyethylene
multilayer bag, in our tissue bank of the Centro Comunitario de Sangre y Tejidos de Asturias. Five hundred and twenty-seven
cancellous bone homografts were obtained from hospitals located 120 km around our Bank. The homografts were submitted to bacteriological
controls and sent to our bank in these bags. They were stored at −70 °C and sent in dry ice to about 50 hospitals, where the
tissue was bacteriologically controlled and grafted. Furthermore, the behaviour of these bags at −140 °C (vapour nitrogen)
or −196 °C (liquid nitrogen) was tested. Our results indicate that Macopharma aluminium-polyethylene bags are suitable for
the transporting and cryopreserving of cancellous bone homografts. These bags could also be used for keeping tissues in nitrogen
containers. |
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Keywords: | Aluminium polyethylene bags Bone homografts Cryopreservation Storage Tissue bank |
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