The induction of a specific form of cytochrome P-450 (P-450j) by fasting |
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Authors: | J Y Hong J M Pan F J Gonzalez H V Gelboin C S Yang |
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Institution: | Laboratoire de Biologie et Technologie des Membranes, C.N.R.S. UM 24 Université Claude Bernard de Lyon, Villeurbanne, France. |
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Abstract: | In previous work we have demonstrated that liver microsomal N-nitrosodimethylamine demethylase (NDMAd) activity is increased in rats by fasting, and we have postulated that this is due to the induction of a specific form of cytochrome P-450. This communication provides evidence for such a hypothesis. Fasting for 24 and 48 h caused 59 and 116% increases, respectively, in NDMAd activity in male rats, and fasting for 48 h caused a 63% increase in female rats. These increases were accompanied by corresponding increases of cytochrome P-450j (P-450ac) determined by immunoblotting. Fasting for 24 and 48 h also increased the mRNA for P-450j by 153 to 250%, as determined by hybridization with a cDNA probe of this cytochrome. The results suggest that fasting affects the gene expression of P-450j. |
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