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Quantitation of chlordiazepoxide and its metabolites in biological fluids by thin-layer chromatography |
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| Authors: | John L. Straughan William F. Cathcart-Rake Don W. Shoeman Daniel L. Azarnoff |
| Affiliation: | Clinical Pharmacology and Toxicology Center, Departments of Medicine and Pharmacology, University of Kansas Medical Center, College of Health Sciences and Hospital, Kansas City, Kan. 66103, U.S.A. |
| Abstract: | Chlordiazepoxide and its 4 major metabolites were assayed after separation by thin-layer chromatography following extraction from biological fluids. The compounds become intensely fluorescent in the presence of red, fuming nitric acid. The resulting compounds are quantitated with a spectrodensitometer with a fluorescent attachment. The sensitivity varies between 0.05 and 0.1 μg. The coefficient of variation is 1.4% for assays in urine and 6.4% in serum. |
| Keywords: | To whom reprint requests should be sent |
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